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Objective:To explore the status quo of job satisfaction, character advantage and subjective well-being of nursing staff, and to explore the relationship among the three, so as to provide theoretical basis for improving the subjective well-being of medical staff.Methods:Using cluster random sampling method, 582 nursing staff in Jinzhou City of Liaoning Province were selected as the survey objects. The General Information Questionnaire, Minnesota Satisfaction Questionnaire, Character Strengths Scale and Subjective Well-being Scale were used to investigate the respondents. The mediating effect of character strengths on job satisfaction and subjective well-being was analyzed by structural equation model. The mediating effect was analyzed and verified by bootstrap method.Results:The score of Minnesota satisfaction questionnaire, Character Strengths Scale and Subjective Well-being Scale of nursing staff were 70.5 ± 10.8, 45.0 ± 6.8 and 24.2 ± 6.3 respectively. The results of correlation analysis showed that there was a positive correlation between the total score of job satisfaction and the total score of character strengths of nursing staff ( r=0.214, P<0.01), and the total score of subjective well-being ( r=0.373, P<0.01). The total score of subjective well-being was positively correlated with the total score of character strengths ( r=0.123, P<0.01). Conclusions:The character strengths of nursing staff plays a mediating role between job satisfaction and subjective well-being.
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Objective:To investigate the correlation between plasma microRNA (miR)-122, miR-33a and the severity of coronary artery disease in patients with type 2 diabetes mellitus (T2DM) and coronary heart disease.Methods:The clinical data of 196 patients with T2DM from January 2019 to October 2021 in Xuzhou First People′s Hospital were retrospectively analyzed. Among them, 81 cases were complicated with coronary heart disease (combined group), 115 cases were not complicated with coronary heart disease (control group). The plasma levels of miR-122 and miR-33a were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction, the plasma level of N-terminal B-type natriuretic peptide precursor (NT-proBNP) was detected by enzyme-linked immunosorbent assay. In combined group, the number of coronary artery lesions was determined according to the results of coronary angiography, and Gensini score was evaluated. Linear regression model was used to analyze the relationship between plasma miR-122, miR-33a and NT-proBNP levels with the incidence of coronary heart disease in patients with T2DM. Receiver operating characteristic (ROC) curve was used to analyze the plasma miR-122 and miR-33a in predicting efficiency of coronary heart disease in patients with T2DM. In combined group, Spearman correlation method was used to analyze the relationship between plasma miR-122, miR-33a and the number of coronary artery lesions, and Pearson correlation method was used to analyze the relationship between plasma miR-122, miR-33a and plasma NT proBNP, Gensini score.Results:The plasma miR-122, miR-33a and NT-proBNP in combined group were significantly higher than those in control group: 5.76 ± 1.35 vs. 1.18 ± 0.33, 1.39 ± 0.37 vs. 0.65 ± 0.11 and (786.87 ± 156.39) ng/L vs. (103.45 ± 19.27) ng/L respectively, and there were statistical differences ( P<0.01). Linear regression result showed that plasma miR-122, miR-33a, and NT-proBNP were positive correlation with occurrence of coronary heart disease in patients with T2DM ( P<0.01); ROC curve analysis result showed that the area under curve of plasma miR-122, miR-33a and combination in predicting coronary heart disease in patients with T2DM were 0.816, 0.845 and 0.912 respectively (95% CI 0.744 to 0.865, 0.768 to 0.892 and 0.836 to 0.967). Coronary angiography result showed that there were 46 cases of single vessel lesions, 25 cases of double vessel lesions and 10 cases of three vessel lesions. The plasma miR-122, miR-33a, NT-proBNP and Gensini score in patients with three vessel lesions were significantly higher than those in patients with double vessel lesions and patients with single vessel lesions: 6.52 ± 0.96 vs. 4.95 ± 0.85 and 3.74 ± 0.52, 1.45 ± 0.31 vs. 1.06 ± 0.25 and 0.81 ± 0.13, (829.78 ± 62.59) ng/L vs. (627.48 ± 47.12) and (502.64 ± 38.24) ng/L, (63.89 ± 12.71) scores vs. (42.18 ± 6.03) and (22.36 ± 2.41) scores, the indexes in patients with double vessel lesions were significantly higher than those in patients with single vessel lesions, and there were statistical differences ( P<0.05). In combined group, Spearman correlation analysis result showed that the plasma miR-122 and miR-33a were positive correlation with the number of coronary artery lesions ( r = 0.879 and 0.825, P<0.05); Pearson correlation analysis result showed that the plasma miR-122 and miR-33a were positive correlation with the plasma NT-proBNP and Gensini score (miR-122: r = 0.896 and 0.788, miR-33a: r = 0.871 and 0.765; P<0.05). Conclusions:The plasma levels of miR-122 and miR-33a are related to the occurrence of coronary heart disease and severity of coronary artery disease in patients with T2DM, which may be used to guide the prevention and treatment of coronary heart disease in patients with T2DM.
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0.05 ). The total effective rate was 89.29 % and 62.50 % in Group A and Group B respectively, the differences being significant ( P
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Objective To explore the possibility of Lipi Huatan Formula (LHF) in inhibit ing the occurrence of early atherosclerosis and its mechanism. Methods Twent y_four rabbits were randomly allocated to Group A (normal control), Group B (mod el control) and Group C (treated with LHF). Rabbit models with early dietary ath erosclerosis were induced by the feeding of fatty food. The morphological featur es and relative thickness of aortic intima, ki_67 expression of vascular smooth muscle cells (VSMCs) and the positive ratio of apoptotic VSMCs were observed by mo rphological, immunohistochemical and TUNEL techniques. Results The relative thickness of aortic intima, ki_67 expression of VSMCs and the positive ratio o f apoptotic VSMCs were decreased in Group C as compared with Group B (P
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[Objective] To observe the in-vitro inductive effect of ginsenosides (GS) on differentiation of rat bone marrow mesenchymal stem cells (MSC) into myocardial cells. [Methods] Marrow stromal cells were isolated from adult SD rats, and then were cultured and cloned by density gradient method and adhesive cultivation to prepare the primary MSC. The cell surface antigens of CD34 and CD44 were detected with flow cytometer to identify MSC. After subculture, the 8th generation of continuous MSC were induced by GS (250 mg/L) and 5-aza (5-azacytidine, 10 ?mol/L) and GS + 5-aza (250mg/L and 10 ?mol/L respectively) for 24, 48 and 72 hours to differentiate to myogenic cells. Meanwhile, a blank control group was set to compare the effect. After the induction, myocardial cell percentage was worked out after examining MSC count and positive cells count under the phase contrast microscope. The specific proteins of Desmin and cardiac troponin I (cTnI) in myocardial cells were detected by immunohistochemistry method, and the cardiac-specific gene expression of ?-MHC (myosin heavy chain) and ?-MHC in myocardiocytes was examined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. [Results] The cultured marrow stromal cells grew well, spindle in shape, and their CD44 expression was positive, indicating that marrow stromal cells differentiated to MSC. After induction with GS and 5-aza, MSC differentiated to myocardial cells, and the myocardial cell percentage was 38% in GS group, 35% in 5-aza group and 39% in GS + 5-aza group, the difference being insignificant. The expression of Desmin, cTnI and MHC in MSC was positive, and MSC were spindle-shaped and looked like fibroblast, indicating that MSC differentiated to myocardial cells. [Conclusion] Ginsenosides can induce MSC to differentiate to myogenic cells in-vitro, and this will supply evidence for cell transplantation.
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0.05). Conclusion Tianma Gouteng Decoction can reverse left ventricular hypertrophy and myocardial fibrosis,and its mechanism may be related to the inhibition of TGF-? 1 expression in cardiac muscle tissue.
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Objective To investigate the effect of Tianma Gouteng Decoction on myocardial fibrosis in renovascular hypertensive rat (RHR). Methods RHR models were established by occlusion of renal artery with artery forceps. The collagen concentration and the contents of collagen type I,III were measured. Meanwhile, the weight of heart and left ventricle was determined. Results (1)Both Tianma Gouteng Decoction and Captopril could reduce the left ventricle weight index (LVI) and collagen contents (P